Recombinant:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
Sequencing:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
Polymerase Chain Reaction:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
Protease Inhibitor:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
Software:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
Cloning:Article Title: DSK2-mediated degradation of F-box protein LAO1 and class I TCPs modulates the nitrogen starvation response.
Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number Arabidopsis: tcp15 Wang et al, 2023 N/A Arabidopsis: tcp14/15 Peng et al, 2015 N/A Arabidopsis: tcp7/14/21 This study N/A Arabidopsis: tcp14/15/ 21 This study N/A Arabidopsis: tcpQ This study N/A Arabidopsis: tcpS Zhang et al, 2018 N/A Arabidopsis: 35S::GFPTCP14/HA-LAO1 This study N/A Arabidopsis: 35S::GFPTCP21/HA-LAO1 This study N/A Arabidopsis: 35S::GFPDSK2/HA-LAO1 This study N/A Arabidopsis: 35S:: HALAO1/ dsk2 cr This study N/A Arabidopsis: lao1 (T)/ atg5-1 This study N/A Recombinant DNA pGEX-4T-1-DSK2CT This study N/A pET32a-TCP7 This study N/A pET32a-TCP14 This study N/A pET32a-TCP15 This study N/A pET32a-TCP21 This study N/A pET32a-DSK2 This study N/A Antibodies Mouse anti-GST Affinity Cat# T0007 Rabbit anti-DSK2 Homemade N/A Mouse anti-His Abmart Cat# M30111 Mouse anti-GFP Abmart Cat# M20004 Rabbit anti-plant actin Easybio Cat# BE0027 Anti-Mouse IgG Peroxidase antibody Sigma Cat# A9044 Anti-Rabbit IgG Peroxidase antibody Sigma Cat# A9169 Anti-HA Roche Cat# 12013819001 Rabbit anti-UB ProteinTech Cat# 10201-2-AP Oligonucleotides and other sequence-based reagents PCR Primers This study Appendix Table S1 Chemicals, Enzymes and other reagents Murashige and Skoog Basal Medium PhytoTech Cat# M519 MS Base Salts (-N, with vitamins) Coolaber Cat# PM1011 Hygromycin B Mei5bio Cat# H61237 DMSO BBI Cat# 67-68-5 protease inhibitor cocktail MCE Cat# HY-K0010 Reagent/Resource Reference or Source Identifier or Catalog Number Magnesium chloride hexahydrate aladdin Cat# 7791-18-6 Glycerol BBI Cat# A600232-0500 Triton X-100 Yeasen Cat# 20107ES76 NP40 Yeasen Cat# 20103ES60 PMSF Diamond Cat# A100754-005 Urea BBI Cat# A510907-002 NaH2PO4 Fisher Scientific Cat# S369 2-mercaptoethanol MACKLIN Cat# CC16419978 NON-Fat Powdered milk BBI Cat# A600669-0250 MG132 MCE Cat# S21619 TBST Fdbio Cat# FD9061 Tris BBI Cat# 77-86-1 SDS BBI Cat# 151-21-3 Glycine BBI Cat# 56-40-5 Cycloheximide beibokit Cat# A1740A TEMED Fdbio Cat# FD2100 IPTG BBI Cat# 367-93-1 Software GraphPad Prism 9 GraphPad Software, USA https://www.graphpad.com/ scientific-software/prism/ ImageJ NIH, USA https://imagej.nih.gov/ij Adobe Illustrator 2023 Adobe N/A Other Anti-HA Affinity Beads SmartLifesciences Cat# SA068001 Anti-GFP Affinity Beads SmartLifesciences Cat# SA070001 Ni-NTA Sepharose 6FF BBI Cat# C600033-0010 Glutathione Agarose Resin Yeasen Cat# 20507ES10 FDbio-Dura ECL FdBio Cat# FD8020 ClonExpress II One Step Cloning Kit Vazyme Cat# C112-01 Plant materials and growth conditions All the Arabidopsis genetic materials used in this study were Columbia (Col-0) ecotype. ..
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